Xpert Ultra Can Unambiguously Identify Specific Rifampin Resistance-Conferring Mutations
نویسندگان
چکیده
منابع مشابه
Rapid detection of rpoB gene mutations conferring rifampin resistance in Mycobacterium tuberculosis.
Multidrug-resistant Mycobacterium tuberculosis strains are widespread and present a challenge to effective treatment of this infection. The need for a low-cost and rapid detection method for clinically relevant mutations in Mycobacterium tuberculosis that confer multidrug resistance is urgent, particularly for developing countries. We report here a novel test that detects the majority of clinic...
متن کاملEffect of rpoB mutations conferring rifampin resistance on fitness of Mycobacterium tuberculosis.
Rifampin is a major drug used in the treatment of tuberculosis infections, and increasing rifampin resistance represents a worldwide clinical problem. Resistance to rifampin is caused by mutations in the rpoB gene, encoding the beta-subunit of RNA polymerase. We examined the effect of three different rpoB mutations on the fitness of Mycobacterium tuberculosis. Rifampin-resistant mutants were is...
متن کاملrpoB mutation conferring rifampin resistance in Streptococcus pyogenes.
Streptococcus pyogenes BM4478 and Staphylococcus aureus BM4479 were isolated from a patient undergoing rifampin therapy. High-level resistance to rifampin was due to the following mutations in the rpoB gene: Ser522Leu in strain BM4478 and His526Asn and Ser574Leu in strain BM4479.
متن کاملRifampin resistance missed in automated liquid culture system for Mycobacterium tuberculosis isolates with specific rpoB mutations.
WHO-endorsed phenotypic drug susceptibility testing (DST) methods for Mycobacterium tuberculosis are assumed to be the gold standard for identifying rifampin (RMP) resistance. However, previous results indicated that low-level, yet probably clinically relevant, RMP resistance linked to specific rpoB mutations is easily missed by some growth-based methods. We aimed to compare the level of resist...
متن کاملFitness cost of chromosomal drug resistance-conferring mutations.
To study the cost of chromosomal drug resistance mutations to bacteria, we investigated the fitness cost of mutations that confer resistance to different classes of antibiotics affecting bacterial protein synthesis (aminocyclitols, 2-deoxystreptamines, macrolides). We used a model system based on an in vitro competition assay with defined Mycobacterium smegmatis laboratory mutants; selected mut...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2018
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00686-18